Entamoeba Genome Project
This project is intended to close E. histolytica, perform comparative sequencing on E. invadens and E. dispar, and identify pathogenicity determinants and host interacting genes. It will deal with twenty-five mega-bases spread across three genomes.
Amebiasis, caused by the parasitic protist Entamoeba histolytica, is one of the most important human parasitic disease world wide [1,2], causing between 40-100,000 deaths annually. Analysis of the sequence data from the recently completed genome project revealed much concerning the basic biology and pathogenesis of the organism and has been a useful resource for the community. However, problems concerning the extreme repetitiveness and high AT-content of the genome resulted in generation of an incomplete, draft assembly of the genome. Here we propose to increase the value of the E. histolytica genome sequence by comparative analysis with sequence data from the non-pathogenic species Entamoeba dispar and the reptilian parasite Entamoeba invadens. We also intend to advance and significantly improve the E. histolytica genome assembly through the generation of a complete physical map and closure of the remaining gaps in the genome.
For more information, including references, please see the project information page.
This project, which started on March 2005, has been split into two parts: the closure of the E. histolytica genome and comparative shotgun sequencing of E. dispar and E. invadens. For statistics on the sequencing completed thus far, please see the statistics page.
Please see the production status page.
Investigators and Collaborators
J. Craig Venter Institute
Mann, Barbara J.
Depts. of Internal Medicine & Microbiology, University of Virginia Health System